Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells

Riferimento: 
Biochem Biophys Res Commun. 2012 May 11;421(3):591-8.
Autori: 
Kim JA, Bae SH, Choi YJ, Kim KH, Park SS.
Fonte: 
Biochem Biophys Res Commun. 2012 May 11;421(3):591-8.
Anno: 
2012
Azione: 
Cellule del carcinoma embrionale F9 (CE) subiscono una differenziazione extra-embrionale (EXE) in risposta al trattamento con l'acido retinoico (RA), che induce l'espressione delle due isoforme (P96 e P67) della proteina adattatore (Dab2) attraverso l'attivazione del fattore di trascrizione GATA-4.
Target: 
RA/carcinoma embrionale.

ABSTRACT
F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.

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