ABSTRACT
The present study tested whether all-trans retinoic acid (ATRA) and 5-Aza-2'-deoxycitidine (5-Aza) affect AML cell differentiation and growth in vitro by acting on the CCAAT/enhancer binding protein α (C/EBPα) and c-Myc axis. After exposure to a combination of these agents, cell differentiation and growth arrest were significantly higher in human and murine MLL-AF9-expressing cells than in MLL-AF4/AF5q31-expressing cells, which were partly associated with increased expression of C/EBPα, C/EBPε, and PU.1, and decreased expression of c-Myc. These findings indicate that MLL-AF9-expressing cells are more sensitive to ATRA and 5-Aza, indicating that different MLL fusion proteins possess different epigenetic properties associated with retinoic acid pathway inactivation.
All-trans retinoic acid combined with 5-Aza-2'-deoxycitidine induces C/EBPα expression and growth inhibition in MLL-AF9-positive leukemic cells
Riferimento:
Biochem Biophys Res Commun. 2012 Nov 16;428(2):216-23.
Autori:
Fujiki A, Imamura T, Sakamoto K, Kawashima S, Yoshida H, Hirashima Y, Miyachi M, Yagyu S, Nakatani T, Sugita K, Hosoi H.
Fonte:
Biochem Biophys Res Commun. 2012 Nov 16;428(2):216-23.
Anno:
2012
Azione:
Acido all-trans retinoico (ATRA) e 5-Aza-2'-deoxycitidine (5-Aza) influenzano la differenziazione e la crescita in vitro delle cellule di leucemia mieloide acuta (AML).
Target:
ATRA-5Aza/leucemia mieloide acuta.
Sostanze: