ABSTRACT
This study was aimed to investigate the proliferation inhibition and apoptosis induction of cucurmosin (CUS) combined with all trans-retinoic acid (ATRA) or arsenic trioxide (ATO) on human acute promyelocytic leukemia cell line NB4. MTT method was used to determine the proliferative inhibition of CUS combined with ATRA or ATO on NB4 cells, and flow cytometry was used to determine the apoptosis induction effect of CUS combined with ATRA or ATO on NB4 cells. Jin's formula was used to assess the synergistic effect of this combinations. The results showed that, compared with single drug, the proliferation inhibitory ratio and apoptotic ratio of CUS combined with ATRA or ATO on NB4 cells was higher than CUS, ATRA and ATO alone. The synergistic index (q) were all larger than 0.85, and the combined effects were significant at low concentrations. It is concluded that the CUS combined with ATRA or ATO synergistically increases the effects of proliferative inhibition and apoptosis induction on NB4 cells.
[Effects of cucurmosin combined with common chemotherapeutics on proliferation and apoptosis of NB4 cells]
Riferimento:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Dec;20(6):1327-31.
Autori:
Xle JM, Liu M, Liu TB, Chen MH, Yang AQ, Yang P.
Fonte:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Dec;20(6):1327-31.
Anno:
2012
Azione:
Cucurmosina (CUS), proteina inattivante i ribosomi, in combinazione con acido all-trans retinoico (ATRA) o triossido di arsenico (ATO) aumenta sinergicamente gli effetti di inibizione proliferativa e induzione all'apoptosi sulla linea cellulare di leucemia promielocitica acuta umana.
Target:
CUS-ATRA-ATO/leucemia promielocitica acuta.
Sostanze: